WLS antibody - 100 µg
Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: G protein-coupled receptor 177
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 46-62 kDa
Uniprot: Q5T9L3
Gene id: 79971
Background: GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).
Field of research: Signal Transduction
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC, IP
Dilution: WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200
Target: WLS
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: G protein-coupled receptor 177
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 46-62 kDa
Uniprot: Q5T9L3
Gene id: 79971
Background: GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).
Field of research: Signal Transduction
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC, IP
Dilution: WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200
Target: WLS
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat